A high-performance third-generation enzyme-linked immunosorbent assay (ELISA) designed for the qualitative detection of IgG antibodies to Hepatitis C Virus (anti-HCV) in human serum or plasma. Utilizes recombinant antigens (Core, NS3, NS4, NS5) to ensure outstanding diagnostic sensitivity and specificity.
Hepatitis C Virus (HCV) is an enveloped RNA virus that causes acute and chronic liver disease. Because early infection is often asymptomatic, screening high-risk groups and blood donations for anti-HCV antibodies is vital to prevent transmission and manage disease progression.
The Genlisa™ HCV (3rd Gen.) ELISA Kit is a solid-phase enzyme immunoassay that detects antibodies against immunodominant regions of the HCV polyprotein. The microtiter wells are pre-coated with highly purified recombinant HCV antigens representing Core, NS3, NS4, and NS5 regions.
If anti-HCV antibodies are present in the sample, they bind to the coated antigens and are subsequently detected using a horseradish peroxidase (HRP) conjugated secondary antibody, producing a colorimetric reaction read at 450nm.
Advanced molecular diagnostic capabilities engineered for clinical excellence and research-grade precision.
Core, NS3, NS4, and NS5 recombinant antigens are utilized to maximize reactivity against diverse HCV genotypes.
Clinical evaluation shows 100% sensitivity and 99.6% specificity, minimizing false-negative and false-positive results.
Incorporates NS5 antigen alongside structural and non-structural targets, improving detection during early infection.
Simple incubation steps (~150 min total) compatible with manual workflows and fully automated ELISA processors.
Validated for use with human serum and common anticoagulated plasma samples (EDTA, Heparin, Citrate).
Positive and negative controls are provided in every kit to validate assay performance and calculation cut-offs.
Designed for diverse diagnostic and research use cases across the clinical spectrum.
Routine screening of blood and plasma donations to prevent transfusion-transmitted HCV.
Aid in the diagnosis of hepatitis C infection in patients presenting with signs of liver disease.
Screening pregnant women to identify maternal infection and manage mother-to-child transmission risk.
Population surveillance and screening in high-prevalence areas or clinical research settings.
| Parameter | Value / Description |
|---|---|
| Technology | Solid-Phase Enzyme-Linked Immunosorbent Assay (ELISA) |
| Generation | Third Generation (3rd Gen.) |
| Antigens Coated | Recombinant Core, NS3, NS4, NS5 antigens |
| Sample Type | Human Serum or Plasma |
| Diagnostic Sensitivity | 100% |
| Diagnostic Specificity | 99.6% |
| Wavelength | 450 nm (reference 620/630 nm) |
| Total Incubation Time | 150 minutes (60 min sample + 60 min conjugate + 30 min substrate) |
| Intended Use | In Vitro Diagnostic (IVD) |
| Storage | 2–8°C |
For in vitro diagnostic use. Contact your regional distributor or ProbGen representative to place an order.
| Product Name | Cat. No. | Pack Size |
|---|---|---|
| Genlisa™ HCV (3rd Gen.) ELISA Kit | GLHCV003 | 96 tests |
| Genlisa™ HCV (3rd Gen.) ELISA Kit | GLHCV004 | 480 tests (5x96) |
Contact our scientific team for technical specifications, bulk pricing, or to request a demonstration of our diagnostics solutions.
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